Ethyl acetate extract of Clausena excavata induces growth inhibition of non-small-lung cancer, NCI-H460, cell line via apoptosis (2024)

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Ethyl acetate extract of Clausena excavata induces growth inhibition of non-small-lung cancer, NCI-H460, cell line via apoptosis References

J Appl Biomed 19:40-47, 2021|DOI:10.32725/jab.2021.007

Ethyl acetate extract of Clausena excavata induces growth inhibition of non-small-lung cancer, NCI-H460, cell line via apoptosis

Shaymaa Fadhel Abbas Albaayit1,*, Mariam Ashfaq Khan2, Rasedee Abdullah3, Mohd Hezmee Mohd Noor4
1University of Baghdad, College of Science, Department of Biology, Baghdad, Iraq
2University of Karachi, International Center for Chemical and Biological Sciences, Dr. Panjwani center for Molecular Medicine and Drug Research, Karachi, Pakistan
3Universiti Putra Malaysia, Faculty of Veterinary Medicine, Department of Veterinary Laboratory Diagnosis, Selangor, Malaysia
4Universiti Putra Malaysia, Faculty of Veterinary Medicine, Department of Veterinary Preclinical Sciences, Selangor, Malaysia

Context: Clausena excavata Burm. f is a plant used in folklore medicine for the treatment of various ailments in South East Asia. The plant parts contain chemical components that are cytotoxic to many cancer cells.

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Objective: The study investigated the cytotoxic effects of ethyl acetate, methanol and chloroform C. excavata leaf extracts on the non-small-lung cancer, NCI-H460, cell line.

Methods: Based on the 3-(4,5-dimethylthiazol-2-yl)-2,5,-diphenyltetrazolium bromide (MTT) assay, among extracts, ethyl acetate C. excavata leaf extract (EACE) was the most potent anti-NCI-H460 cells, with IC50 value of 47.1 ± 6.1 μg/ml. The effects of EACE on NCI-H460 cells were also determined by clonogenic, 4', 6-diamidino-2-phenylindole (DAPI), and annexin-V-fluorescein isothiocyanate/propidium iodide-PI flow cytometric assays. Reactive oxygen species (ROS) production and apoptotic gene expressions was determined via flow cytometry and real-time quantitative PCR, respectively.

Results: EACE-treated NCI-H460 cells after 48 h underwent apoptosis as evident by loss of cell viability, cell shrinkage, and chromatin condensation. The results also showed EACE mediated increase in ROS production by the NCI-H460 cells. After 48 h treatment, EACE increased the pro-apoptotic BAX and decreased the anti-apoptotic Bcl-2, Survivin and c-Myc gene expressions.

Conclusions: EACE is a potential anti-lung cancer by increasing cancer cell ROS production and apoptosis.

Keywords: Apoptosis; Cytotoxicity; ROS; Survivin
Conflicts of interest:

We wish to confirm that there is no known conflict of interests associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.

Received: April 4, 2020; Revised: November 13, 2020; Accepted: December 9, 2020; Prepublished online: March 1, 2021; Published: March 3, 2021 Show citation

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Fadhel Abbas Albaayit S, Khan MA, Abdullah R, Hezmee Mohd Noor M. Ethyl acetate extract of Clausena excavata induces growth inhibition of non-small-lung cancer, NCI-H460, cell line via apoptosis. J Appl Biomed. 2021;19(1):40-47. doi:10.32725/jab.2021.007. PubMed PMID:34907714.

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